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Evaluation of antioxidant capacity of SAC DPPH Free Radical Scavenging Capacity Assay Kit (Nanjing Jiancheng Bioengineering Institute, A153-1-1, Nanjing, China): Prepare the working solution according to the kit instructions, mix with different concentrations of SAC solution, incubate in the dark at 25 for 30 min, centrifuge at 4000 rpm for 5 min, transfer 100L to a cuvette, zero with 80% methanol, and measure absorbance at 517 nm

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Refrigerator was accidentally set too cold If your refrigerator temperature dropped below 0 degrees Celsius and the reconstituted solution partially froze, treat it as a freeze event and discard