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How to tell if peptides have degraded Visual inspection (reconstituted) Fresh peptide solution should be : Clear (transparent) Colorless (or very slight tint) No particles or cloudiness No discoloration Signs of degradation : Cloudiness or haziness Yellow or brown tint Visible particles floating Crystallization Oily film on surface If any of these present : Don't use, peptide has degraded

Signaling profiles in HEK 293T cells co-expressing GLP-1 and GIP receptors

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Steric constrains were introduced