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doi: 10.1016/0305-0491(95)02138-8 44 JiangT.YinF.YaoJ.BrintonR

PYL4-mCherry halved the LUC signal and reduced the YFP fluorescence of the DWA1RGL2 complex by ~70% compared with mCherry alone (Fig

Finally, whole cell fractions and beads were either stored at 20 C or denaturised (LDS Sample buffer, 3% -mercaptoethanol, 20 mM DTT (Thermo Fisher, 20290), 2 mM biotin, 10 min at 95 C) and resolved by SDS-PAGE