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NTDs include various malformations, such as lesions of the brain (e.g., anencephaly, encephalocele) or lesions of the spine (spina bifida), which are devastating and usually incompatible with life (57)

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To overcome these shortcomings, nanoscale drug delivery systems (NDDSs), such as polymer nanoparticles (NPs), have been introduced to maximize the efficacy of DHA in single or multiple drug therapies (Wong et al., 2022)

4.2 Narrative summary 4.2.1 Description of the type of intervention in the included studies and how they were implemented The type of intervention used is HMGB1, the ubiquitous and highly evolutionarily conserved nuclear protein, which was tested in experimentally induced DCM to elicit a cure phenotype from this rodent model