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doi:10.1016/j.amjcard.2020.06.043 Nijjar PS, Connett JE, Lindquist R, et al

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Subsequently, cultures expressing either PgAFP or AfAFP were scaled up to 4 L of fresh LB medium with 100 g/mL ampicillin and maintained under identical conditions until the optical density at 600 nm reached 0.5