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That gap widens over time, from roughly 4-5 percentage points at 24 weeks to over 6 percentage points by 68-72 weeks

RPMI/B27 medium (61,870,036), B27 supplement (17,504,044), GlutaMax supplement (35,050,061), Insulin- Transferrin-Selenium supplement (41,400,045), Knock Out DMEM (10,829,018), KnockOut Serum Replacement (10,828,028), Fetal Bovine Serum (A3840002), MEM Non-Essential Amino Acids Solution (11,140,050), Geltrex (A1413302), Dulbeccos phosphate-buffered saline, calcium and magnesium free DPBS / (14,190,144), ProLong Gold Antifade Mountant with DAPI (P36935), Trypsin (90,057), Trizol Reagent (15,596,026) were from Thermo Fisher Scientific (Rockford, IL)

The number of macroscopic colonies in the spleen was counted after 12 days, following fixation in Bouins solution [2]

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