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The cells were lysed by ultrasound for 20 min at 4 C, followed by centrifugation at 12,000 rpm for 15 min to collect the supernatant

Co-culturing ESCs with monocyte-derived macrophages and NK cells from endometriosis patients showed that the interaction between ESCs and macrophages downregulates NK cell cytotoxicity by enhancing the release of IL-10 and TNF- (37), potentially allowing endometrial fragments to evade immune clearance (38)

Microglial NF-B activation under GSH-depleted conditions creates a pro-inflammatory loop where increased ROS promotes NF-B activation, which upregulates inflammatory cytokines and further stimulates oxidative stress (Liu et al., 2017

DNA DAMAGE As of 2012, 145 studies had shown an association between mitochondria function and autism