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These rapid changes in organization cause cells to experience stretch, pressure, and shear stress, which cause mechanoactivation of various proteins, such as cadherins, caveolins, Rho, and cdc42, and aid in development in normal physiological conditions ( vegfr-2 in a genetically-modified mouse model is embryonically lethal by stage E10.5 and showed no vasculature, suggesting that VEGFR-2 is required for vasculogenesis in development ( Angiogenesis in Tissue Engineering While there has been a significant rise in interest for tissue engineered scaffolds for replacement of disease tissues or organs in recent years, generation of large-scale systems has been limited by size restrictions due to limited nutrient diffusion

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The key takeaway is that you, the researcher, are in control of the final concentration