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Skin penetration, plasma binding, and copper-pool dynamics complicate dose extrapolation

Available from: Mann, J.F.E, &Flack, J.M

In certain embodiments, the subject may be a subject having a baseline intracellular level of GSH, tGSH, or both, when compared to children without a diagnosis of autism (it has previously been found that plasma, intracellular, and even brain levels of GSH may be decreased compared to control children without autism

In the methods described herein, a mixture of lipids is combined with a buffered aqueous solution of nucleic acid to produce an intermediate mixture containing nucleic acid encapsulated in lipid particles, e.g., wherein the encapsulated nucleic acids are present in a nucleic acid/lipid ratio of about 10 wt % to about 20 wt %