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For quantification, the images were counted manually or automatically using the ImageJ plug-in Cell Counter. Viability assay Differentiated neurons were passaged and plated at a concentration of 40,000 cells per well to a 96-well culture plate

Cell Metab 37, 857869.Google Scholar Lautrup, S, Sinclair, DA, Mattson, MP et al

doi: 10.5507/bp.2016.044 113 PanH.YangS.KulyarM

In recent years, numerous studies have shown that the high-altitude hypoxic environment causes nonnegligible damage to various organs and systems of the body