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To each pellet, 1 mL of scintillation fluid was added and the cell suspension was filtrated on nitrocellulose Whatman filters using a vacuum filtration box (Hoefer, USA)

Attempts to establish and validate a method to determine local tissue levels by microdialysis have been reported in adolescents with type 1 diabetes, where endocrine levels are a poor marker of local IGF-I activity (115)

TABLE 1 Summary of main effects of microbiome-based interventions in NDDs

To overcome this shortcoming, a combination of 3-BP and another drug, which induced a synergistic antitumor effect and decreased 3-BP doses, might overcome these drawbacks