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As attempts to answer these questions, the present study is firstly aimed to determine whether the key hepatic enzymes and the redox-active metal ion involved in metabolic regulation [such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and the ferrous ion (Fe 2+ )] would significantly be improved in animals suffered from CSD and received ALA treatment

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Based on KEGG annotation, 1,484 genes were assigned to 40 functional categories, with major groups including overview maps (284 genes), carbohydrate metabolism (120 genes), and membrane transport (98 genes), as illustrated in Figure 6D