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Description

Total RNAs were extracted by TRIzol Reagent (Invitrogen, catalog number 15596026)

1 ml of pre-cooled methanol/water (80:20 v/v) was added to each well and plates incubated at 80 C for 30 min

Strength training, daily walking (10,000 steps), and a protein-rich diet are essential to physically burn off the triglycerides that the peptide commands your fat cells to release