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Sperm take around 64 days to develop from early precursor cells to mature, motile sperm, with some estimates extending the range further, so anything he changes in the few weeks before egg collection makes little difference to the cells used in that cycle

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doi: 10.1016/j.numecd.2019.07.012 15 FathizadehHMilajerdiAReinerKolahdoozFChamaniMAmiraniEet al

JC-1 dye accumulates in active mitochondria, leading to the formation of J-aggregates with red/orange fluorescence emission at 590 nm, while under conditions of mitochondrial depolarization or low potentials, JC-1 exists as J-monomers emitting a green fluorescence at 525 nm