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Similar to other measures reported via the HQR System for the PCHQR program, we estimate a burden of no more than 10 minutes per hospital per year, as each hospital would only be required to report one aggregate numerator and denominator for all patients

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26S proteasome activity assay Briefly, pulverized liver samples (20 mg aliquot) were homogenized in 26 S proteasome lysis buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1 mM ethylenediaminetetraacetic acid (EDTA), 5 mM magnesium chloride (MgCl 2) [pH 7.5]) with a hand-held Potter-Elvehjem homogenizer

Statistical significance was calculated using Dunnetts T3 multiple comparison