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425 , 479494 (2000) Barres, B

Dissolve the mixture of A and B in a solvent in which they are both soluble when hot and relatively insoluble when cold, filter off any undissolved B, and cool slowly

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Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation