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ANTIBIOTICS-BASEL 10 (1), 49

Cells were lysed in ChIP lysis buffer (50 mM TrisHCl pH 8.0, 10 mM EDTA, 1% SDS, and protease inhibitors), sonicated to obtain 200400 bp chromatin fragments, and incubated with anti-Flag M2 agarose (pre-coated with 0.1 mg/mL BSA and sheared salmon sperm DNA) at 4 C overnight