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We discovered that Diphenyleneiodonium chloride (DPI), an inhibitor of flavoprotein enzymes and mitochondrial complex I, synergizes with mitoquinone mesylate (MitoQ), a mitochondria-targeted antioxidant in 2D and 3D in vitro models of NB

Despite their small size, they have immense biological significance in molecular biology: peptides function as a peptide hormone like PPY, enzymes, neurotransmitters, and signal molecules that mediate immune responses, cell signaling, tissue repair, and metabolic regulation

Chemiluminescence was detected using SuperSignal West Dura Substrate (Thermo Fisher Scientific, Waltham, MA), followed by autoradiography