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Finally, in rats fed fructose for 45 days to produce a non-obese insulin resistance state, there were reductions in muscle BCAT2 activity (15%), even though activities of the enzyme in liver and adipose tissue were not affected ( Interestingly, supraphysiological supplementation of metformin (2 mM), a commonly prescribed drug for T2DM, for 12 h increases BCAT2 mRNA expression levels (30%) but significantly reduces it after 24 h in C2C12 myotubes