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Significance was determined by unpaired two-tailed Students t -test ( e , f ) and two-way ANOVA with Tukeys multiple comparison test ( h , i )

Prior to BCA quantification, the samples were diluted 50-fold to reduce the potential background from Urea, SDS, and DTT

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Research suggests that the Warburg effect not only supports tumor cell growth but also suppresses immune cell function through changes in the TME metabolic milieu (e.g., lactic acid and hypoxia), thereby increasing resistance to immune checkpoint inhibitors (ICI) and chemotherapeutic agents (Zh et al., 2023