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Description

This creates a dependency dynamic where continued use is necessary to maintain results

For explant assays, the tissue (SMG and PG) was incubated with 50200 nM CCh (200 nM CCh shown in results) for 4 h before being lysed for RNA

Documentation and tracking Keep a simple log for each vial: reconstitution date, water volume added, concentration, and a running tally of doses drawn with dates