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Cellular Biochemistry 122, 19581967

Proteins were transferred to a nitrocellulose membrane using a semidry transfer system (Bio-Rad) in transfer buffer (25 mM Tris, 150 mM glycine, and 20% MeOH) for 2 h at 15 V

Published: February 12, 2014 This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose

Recent studies with ASCT2 knockout cells reveal that several compensatory mechanisms compensate for its absence, and that it nonetheless plays a role in tumorigenesis in ways that remain to be elucidated