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Bodipy 493, a cell permeable nonpolar dye, accumulates in neutral lipid droplets and fluoresces when bound to neutral lipids 43,44

2018;48:e20170176

Formation of these latently infected cell reservoirs is started early during the HIV-1 infection in diverse cell types and multiple anatomical sites with limited drug penetration.18 Thus, HIV-1 infection turns into an incurable disease because the integrated replication-competent proviruses become invisible to the immune system and not susceptible to ART.18 Furthermore, virus production, disease progression and virus spreading can rapidly develop after cessation of ART.19 Thus, it is not surprising that these viral reservoirs are one of the main obstacles to achieve a definitive HIV-1 cure

For the LC-MS aliquot, 50 L of acyl-CoA internal standard was added