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2.3 Protein Extraction and Digest Human serums, mixed in each group, were prepared by centrifugation at 4,000 g for 10 min and removed interfering high-abundance proteins of serum by the Agilent Multiple Affinity Removal Column (Multiple Affinity Removal Column, 4.6 mm 50 mm

Coimbra, [email protected] This article was submitted to Inflammation, a section of the journal Frontiers in Immunology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Despite their small size, they have immense biological significance in molecular biology: peptides function as a peptide hormone like PPY, enzymes, neurotransmitters, and signal molecules that mediate immune responses, cell signaling, tissue repair, and metabolic regulation

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