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Hematoxylin and eosin staining and immunohistochemistry Cytotoxicity detection Statistical analysis t -test or one-way ANOVA

FIGURE 3 IL-13 DRIVEN INFLAMMATION IS UNAFFECTED BY GGsTop, BUT INDUCTION OF MUCOUS EXPRESSION IS ATTENUATED Compared to control lung, exposure to IL-13 induced the level of lung mRNA transcript expression for IL-33 by sixfold ( Figure 3B ) and recruited eosinophils to the lung in the absence ( Figure 3C ) and presence ( Figure 3D ) of GGsTop