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Mitchell et al.[6] measured the level of GSH depletion in the liver (of mice), and the amount of covalent binding of radiolabeled APAP metabolites (later identified as NAPQI) in the liver after a wide range of doses of APAP

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This mechanism plays a critical role in the cellular response to oxidative stress and the regulation of antioxidant gene expression, and it may also be significant in disease progression [148]