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After 3 months of dietary intervention, behavioral tests showed that mice on the KD performed significantly better on the rotarod compared to control groups, regardless of their specific genotype (213)

For functional detection, PC12 cells were transfected with three distinct shRNA sequences targeting the NUDT1 gene, all of which successfully knocked down NUDT1 expression, as confirmed by both qPCR and Western blot analyses (Fig

Interestingly, given the importance of butyrate in the maintenance of the intestinal barrier, IBS has been associated with a reduction in butyrate producing gut micro-organisms (Pozuelo et al., 2015)

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