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To model 250 mcg: 250 5,000 = 0.05 mL, and 0.05 100 = 5 units on the syringe

Cells were treated with different concentrations (from 1 to 1000 M) of a NO synthesis inhibitorL-NAMEduring different times (12, 24, 48, 72, 96, and 120 h), with and without retreatment every 24 h (retreatment was applied to approximate this in vitro model to the chronic exposure that occurs in vivo)

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Folate has the capacity to impede the development of cancer cells by virtue of its involvement in one-carbon metabolism, which encompasses the regeneration of methionine from Hcy, the synthesis of S-adenosylmethionine (SAM), the regulation of DNA methyltransferase, and the facilitation of thymidine synthesis [135]