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Description

Structural reanalysis of pNp and Np (Protein Data Bank [PDB] entries 7ZU8, 7ZVA, 7ZVB, 7ZVC (del Amo-Maestro et al, 2022)) identified two disulfide bridges (C 219 C 224 and C 358 C 379

These data were later confirmed in a study in vivo on a pig model

The second method is based on the metabolic labeling of S-palmitoylated proteins in live cells with palmitate analogues containing an azide or alkyne group, and thus will reveal the proteins that have undergone S-palmitoylation during the labeling time