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Activation of these pathways leads to the production of cytokines and the activation of various inflammation signaling pathways within the host (55).Although some moderate activation of the immune system will lead to increased therapeutic efficacy when used to modulate an individual's metabolic disease (14), excess activation or stimulation could lead to strong inflammatory response or worsening of autoimmune disease (39) (75).In addition, immune system responses could also be stimulated due to exposure to heterologous proteins produced by genetically engineered probiotics, such as GLP-1 analog, IL-10 (14) (19)