mitochondrial glutathione redox potential Glutathione: Recent Insights and Role in Disease Selective Disruption of Mitochondrial Thiol

Formation of intermolecular disulfide bonds mediates association of the two subunits (Seelig et al., 1984

Sections were then incubated overnight at 4C with primary antibodies against Iba1 (1:300, Proteintech), diluted in blocking buffer

Without rapid reperfusion, this profound pump failure progresses with further ventricular dilation and continued decline in cardiac output, ultimately resulting in irreversible cardiogenic shock , characterized by severe end-organ hypoperfusion, lactic acidosis, and hemodynamic collapse

The mitochondria were washed twice with isolation buffer and centrifuged at 12,700 g for 5 min at 4 C