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During homeostasis, diverse cytosolic and extracellular DNases eliminate self-DNA that appears outside the nucleus and in addition, the cytoplasmatic expression of dsDNA sensors was believed sufficient to avoid erroneous immune responses to self-DNA: dying cells will usually release their DNA into the extracellular space, and DNA being a heavily negatively charged molecule does not normally pass through membranes

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Glutathione peroxidase activity was assayed by the method of Chiu et al

The molar ratios of bound C*-PTDH and C*-GFP to NC1A-CBD, as determined from SDS-PAGE analysis, are 0.35 and 0.92, respectively