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For RT-qPCR, 1 l of cDNA was combined with Master Mix Green (RealQ Plus 2x, Ampliqon, Denmark) and gene-specific primers, and the reactions were performed on RT-qCR system (CFX96 Bio-Rad, USA)

We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49 , the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, which indicates the mTORC1 activity

To assess daily selenium intake, two non-consecutive 24 h food recalls were performed using the ASA24 Dietary Assessment Tool (National Cancer Institute) [31]

In the US, an estimated 42 percent of the population are deficient in vitamin D