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DAPI used for nuclear staining was procured from Invitrogen-Molecular Probes (Carlsbad, CA)

These reactions in immune cells affect sirtuin activity by modifying the NAD pools

Studies have also shown that enhancing HIF-1 expression in adipose-derived stem cells (ADSCs) improves both osteogenic and angiogenic capacities, highlighting HIF-1 as a key transcriptional regulator of cellular function via the HIF-1/VEGF/AKT/mTOR pathway (Song S

Availability of data and materials The raw sequence data, corresponding metadata, quality filtered reads, representative sequences and taxonomy assignments to the representative sequences of this study are available in figshare.com ( Ethics declarations Ethics approval and consent to participate Goats that were donors of rumen fluid were maintained according to the ethics committee and Institutional Animal Care and Use Committee standards for the Nanjing Agricultural University