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After brief sonication of the PreserveX-QML/membrane mixture, we detected a significant FRET signal at 585 nm, indicating efficient intermixing of PreserveX-QML with the membrane components ( Figure 3 )

Consequently, an extracellular cystine or intracellular cysteine deficiency or inhibition of system ( SLC7A11 ) would reduce the GSH level, inhibit GSH-dependent GPX4 activity, and lead to the intracellular accumulation of L-ROS and cell death (Dolma et al., 2016

Treatments with free ligands at 5 mM concentration had not only no toxic effects but even improved the impedance of the cell layers (Fig