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service members who were exposed to significant blast and who met the criteria for mTBI were offered to participate in the study, and 81 individuals agreed

C57BL/6J-TgH ( Bnip3 / ) 13 and heterozygous crossed Bnip3-3xFlag-knockin mice ( Flag-Bnip3 ki/ki , Flag-Bnip3 ki/+ , Flag-Bnip3 / ) were bred and maintained in the local animal house of the University Hospital Essen

Fractional enrichment in liver cysteine was not detectable due to the low cysteine pool in mice fed a HF-SAAR diet

Commercial MR diets contain 0.17% methionine compared with normal levels of 0.86%, but notably, the MR diets also lack cystine 8,9 , another sulfur-containing amino acid (SAA), which is required for protein synthesis, including synthesis of glutathione, taurine, CoA and ironsulfur clusters 10,11