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The complex was concentrated to 0.5 ml using Ultra Centrifugal Filter (ThermoFisher MWCO, 100 kDa) and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 5 M ligands, 0.00075%(w/v) LMNG, 000025% glyco-diosgenin (GDN, Anatrace) and 0.00015% (w/v) CHS to separate complex from contaminants

A Third Worked Example (Uneven Split) Not every 10 mg blend is a 5 + 5 split

Keeping these forms in balance is extremely important for maintaining health

This mislocalization increases cytosolic PUFA esterification, generating lipid peroxides that promote ferroptosis in neighboring epithelial cells, reinforcing the immune-epithelial crosstalk within the axis