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This SMD corresponds to a moderate effect size

Until now, the tested carbon-containing nutrient metabolites include amino acids, sugars, nucleotides, and peptides

As proxies for liver damage, the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in serum samples using Alanine Aminotransferase Activity Assay Kit (MAK052, Sigma-Aldrich) and Aspartate Aminotransferase Activity Assay Kit (MAK055, Sigma-Aldrich), respectively, according to the manufacturers instructions and adding 3 L and 5 L of serum per well for ALT and AST activity, respectively

After inhalation anesthesia with isoflurane was administered to pregnant rats at 20 th day of gestation, cesarean section was performed to remove the placental tissue and then suture the incision