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Cross talk between eIF2alpha and eEF2 phosphorylation pathways optimizes translational arrest in response to oxidative

Cells were seeded in a 24-well plate at a density of 2x106 cells/ml in a complete medium consisting of RPMI 1640 (Gibco, Grand Island, NY), 10% fetal calf serum (Atlanta Biologics, Atlanta, GA), 100 U/ml of penicillin G sodium (Gibco), 100 g/ml of streptomycin sulphate (Gibco), 0.25 g/ml of amphotericin B (Gibco), and 2 mM L-glutamine (Gibco).The following in vitro treatments were conducted in triplicate wells for each animal: PBS (negative control), pokeweed mitogen (10 g/ml

Caloric restriction mimicry: Some investigators have noted that the metabolic effects of glutathione repletion in older adults improved insulin sensitivity, reduced inflammation, enhanced mitochondrial function overlap substantially with known benefits of caloric restriction, suggesting that glutathione deficiency may partly mediate the metabolic decline of aging [10][11]

While not common everywhere, its an option for patients who dont do well with shots or have trouble absorbing vitamin B12