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Then, they were rinsed with phosphate buffered saline (PBS), sealed with buffered glycerol, and finally observed under fluorescence microscopy

[PubMed] [Google Scholar] Yoshida K, Hirokawa J, Tagami S, Kawakami Y, Urata Y, Kondo T

Neurobiology of Disease

Next, 3,3,5,5 Tetramethylbenzidine (TMB) substrate is used to enable visualization of the reaction and colour develops in proportion to the amount of rat p-IGF1R