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Hydrogen peroxide solution was substituted by phosphate buffer in the negative control

They may be utilized as induction or debulking therapy to lower the urate pool, followed by maintenance with urate-lowering therapy (ULT)

After 48 h, cells were washed with ice-cold PBS and lysed with 700 l of non-denaturing DISC lysis buffer (30 mM Tris-HCl (pH 7.4), 120 mM NaCl, 2 mM EDTA, 2 mM KCl, 1% Triton X-100, Roche complete protease inhibitor cocktail, 10 M PR619 and 1 mM NEM) on ice for 30 min

The amino acid sequences of these polypeptides differ from the naturally occurring proteins corresponding to the markers encompassed by the present invention, but retain biological activity