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Fraction 4 was subjected to preparative reversed phase HPLC over a Luna PFP(2) (pentafluorophenyl) column (Phenomenex, 250 mm 21.2 mm, 5 m) using a gradient of 70:30 to 100:0 CH 3 OHH 2 O (10 mM of ammonium acetate in both phases) over 20 min at a flow rate of 20 mL/min

Mild irritants can trigger a disproportionately large irritation of the skin (burning and itching) that then can result in prolonged redness and darkening of the skin

In addition, these peptides may indirectly regulate AMPK activity to promote mitochondrial efficiency and FoxO transcription factors (Table 2)