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In a double-blind, placebo-controlled trial in 11 smokers and 13 nonsmokers given -tocopherol and -tocopherol that was labeled with deuterium, supplementation with vitamin C reduced the rate of vitamin E loss in plasma, likely by regenerating tocopheryl radicals back to nonoxidized forms (22)

These interactions provide a structural basis for the previous mutagenesis results of IGF1 showing that the Y31A mutation of IGF1 reduces its receptor-binding affinity by six fold 21

The pooled fractions (approximate 6 ml) were concentrated (approximate 0.6 ml) (centrifugal tube: Amicon Ultra-4 centrifugal filter unit with 10 kDa cutoff, from Millipore) and followed by 2D crystallization trials

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