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Table 2: Cohort 1 (n = 71,580) Costs of Oral Supplements and Intramuscular Injections

NAD+ Metabolism and Salvage Pathway Research Laboratory studies investigate effects on cellular NAD+ levels: NAD+ Quantification: Measurement of total NAD+, NADH, NAD+/NADH ratio in cells/tissues Salvage Pathway Flux: Investigation of nicotinamide NAD+ conversion via NAMPT NAD+ Precursor Effects: Comparison to nicotinamide riboside (NR), nicotinamide mononucleotide (NMN) supplementation Tissue-Specific Effects: Examination of NAD+ changes in liver, adipose, muscle, other tissues Temporal Dynamics: Studies on time course of NAD+ elevation following NNMT inhibition Research examines whether NNMT inhibition represents an alternative strategy for enhancing cellular NAD+ beyond direct precursor supplementation

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