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Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1

By directly scavenging reactive oxygen species (ROS), it helps to prevent mitochondrial dysfunction, supports ATP synthesis, and inhibits the pathways leading to cell death, including apoptosis

79 In fact, an analysis of the iPSC and stem cell transcriptomes showed highly upregulated levels of NAMPT

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