Sample Preparation and Label Free Quantitative Proteomic Analysis Cells were lysed using lysis buffer (4% SDC in 0.1M Tris-Hcl, ph = 8.0) with SIGMAFAST Protease Inhibitor (Sigma, USA)
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acnes , reducing the release of harmful substances such as bacterial endotoxins while promoting the production of beneficial metabolites like short-chain fatty acids
