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Each measurement was repeated three times

Mutations in the rrs A1401G gene and phenotypic resistance to amikacin and capreomycin in Mycobacterium tuberculosis

After centrifugation and removal of the supernatant, cells were subjected to hypotonic treatment with a solution of 0.075 M KCl for 30 min at 37 C, followed by fixation in a 3:1 methanol to acetic acid solution

While dangerous interactions have not been documented, combining multiple GABA-affecting substances increases sedation risk and should be avoided or carefully monitored