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siRNA-mediated RNA Interference For RNA interference, cells in 6-well plates were transfected with designed siRNAs (an irrelevant sequence [control] siRNA), GSTP1 siRNA-1 and GSTP1 siRNA-2, each at 5 nmol/L, using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) for 48 h according to the manufacturers instructions

Therefore, gut microbiota may directly contribute to the prolonged sequelae of COVID-19, suggesting its potential as a therapeutic target (Mendes de Almeida et al., 2023) (Table 2)

Vodicka P, Vodenkova S, Danesova N, et al

A study further revealed that Bifidobacterium activates the hepatic farnesoid X receptor (FXR) pathway, thereby downregulating lipogenic gene expression and reducing endotoxin translocation to the liver, suggesting an emerging direction for metabolic dysfunction-associated steatotic liver disease management