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To maintain physiological conditions, the pH of fresh whole blood was stabilized by the addition of 5% (v/v) (for monkey, rat, and mouse) or 7% (v/v) (for human and dog) phosphate buffer pH 7.4 (70 mM), which was considered during evaluation

The function of the fatty-acid oxidation pathway in fibroblast cultures and biomarkers for abnormalities in non-mitochondrial fatty-acid metabolism were not consistently abnormal across the subgroup of ASD children, consistent with the notion that the abnormalities in fatty-acid metabolism found in this subgroup of children with ASD were secondary to TCAC abnormalities