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The myelinated area was detected under an Olympus light microscope (Olympus, Tokyo, Japan), photographed, and quantified as percentage using ImageJ analysis software at 400x magnification by an expert pathologist who is blinded to the experiment

We then overexpressed the dominant negative form (H79G) of SAR1A, a GTPase important in forming COP II vesicles (28)

While we found increased levels of BCAAs, histidine, histamine, and anserine in KOA IFP, the calculated BCAA/histidine ratios did not reach statistical significance

Low Vitamin D Levels: Individuals with low vitamin D3 levels (below 40 ng/ml) are at significant risk for developing chronic inflammation and autoimmunity (10)